WebMar 28, 2012 · Comparative structural analyses between iLOV and its progenitors reveal mutation-induced constraints in the environment of the flavin mononucleotide (FMN) chromophore; in iLOV, the methyl group … WebOct 16, 2024 · In the C-terminal light-oxygen-voltage (LOV) domain of plant phototropins (LOV2), blue light activation leads to formation of an adduct between a conserved Cys residue and the embedded FMN chromophore, rotation of a conserved Gln (Q513), and unfolding of a helix (Jα-helix) which is coupled to the output domain.
Phototropin - an overview ScienceDirect Topics
WebNov 18, 2004 · Each LOV domain binds one flavin mononucleotide (FMN) chromophore (reviewed in Briggs and Christie 2002; Liscum et al. 2003 ). The absorption spectrum of the FMN-containing LOV domain indicates that the FMN is in its fully oxidized state in darkness. WebOct 17, 2008 · With regard to LOV2 domain function, the absorbance spectra of dark-equilibrated proteins showed clear evidence of a 447-nm peak consistent with a noncovalently bound FMN chromophore , and light activation of all the chimeric enzymes triggered a characteristic spectral shift to a 390 nm–absorbing species due to formation … gps wilhelmshaven personalabteilung
Oxidation of alkyl benzenes by a flavin photooxidation …
WebAug 8, 2000 · Cytochrome P450BM-3 is a self-sufficient bacterial protein containing three naturally fused domains which bind either heme, FMN, or FAD. Resolution of protein and … WebBoth LOV1 and LOV2 undergo a self-contained photocycle, which involves the formation of a covalent adduct between the FMN chromophore and a conserved active-site cysteine residue (Cys39). Replacement of Cys39 with alanine abolishes the light-induced photochemical reaction of LOV1 and LOV2. WebJan 31, 2024 · Through this multifaceted approach, we show that Q513 and N414 are critical mediators of protein structural dynamics, linking the ultrafast (sub-ps) excitation of the … gps wilhelmshaven