http://www.scholarpedia.org/article/Fluorescent_proteins Fluorescent proteins are quite versatile and have been successfully employed in almost every biological discipline from microbiology to systems physiology. These ubiquitous probes have been extremely useful as reporters for gene expression studies in cultured cells and tissues, as well as living animals. In … See more Osamu Shimomura and Frank Johnson, working at the Friday Harbor Laboratories of the University of Washington in 1961, first isolated a … See more Among the most important aspects of the green fluorescent protein to appreciate is that the entire 27 kiloDalton native peptide structure is essential to the development and maintenance of … See more Illustrated in Figure 1 are two examples of multiple fluorescent protein labeling in living cells using fusion products targeted at sub-cellular … See more Within the hydrophobic environment in the center of the green fluorescent protein, a reaction occurs between the carboxyl carbon of Ser65 and the amino nitrogen of Gly67 that results in … See more
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WebBy Jason Niehaus. Luciferases are a class of enzymes capable of catalyzing chemical reactions in living organisms resulting in the emission of photons. The most familiar bioluminescent organism for most people is the firefly ( Photinus pyralis) and perhaps not surprisingly it is also the most commonly used bioluminescent reporter. This beetle ... WebJan 4, 2016 · Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. slug test interpretation
Background-protein effects on fluorophotometric data - PubMed
WebBackground fluorescence of common membranes. Common membranes such as PVDF have high levels fluorescence in the low visible light range (488 nm) whereas specialty low-fluorescence PVDF does not. Exposure … WebFluorescent labeling is the process of covalently attaching a fluorophore to another molecule, such as a protein or nucleic acid. This is generally accomplished by using a … WebGFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins … solahart gold coast