Fl83b cells

Author: thrg

August undefined, 2024

WebNormal mouse hepatocyte cell line FL83B (ATCC CRL-2390) was cultured in Ham’s F-12 K medium containing 10% fetal bovine serum (FBS), 100 mg/mL penicillin and 0.25 mg/mL …

Nontumorigenic Mouse Hepatocyte Cell Lines Fl83b

WebJan 15, 2024 · FL83B cells were induced as fatty liver cell model by 0.5 mM oleic acid for 48 h, and treated with various concentration of fucoxanthin for 24 h. The results demonstrated that fucoxanthin significantly suppressed lipid accumulation and decreased lipid peroxidation in hepatocytes. WebThe short-and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture system were determined (Fig. 1A), which could monitor cellular morphology ... ph of body

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WebJul 1, 2024 · 2.2. Cell line and treatment. FL83B cells purchased from the Bioresource Collection and Research Center (BCRC, Taiwan). Cells were cultured in F12 medium (Invitrogen-Gibco™, Paisley, Scotland) added with 10% FBS, 2 mM glutamine, 1% penicillin and streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2.Cells … WebMay 1, 2024 · FL83B cells were seeded on 6-well plates and incubated with 0.5 mM oleic acid for 48 h. The cells were treated with or without tomatidine (0–10 μM) for 24 h. The plate was washed and the cells fixed with formalin solution before staining with Oil Red O solution to assay oil droplets as described previously [25]. Using an inverted microscope ... WebJan 12, 2024 · FL83B cells were co-transfected with pDR1 and pRL-TK (Promega, Seattle, WA, USA) containing CMV promoter-controlled Renilla luciferase gene using fuGENE HD (Promega, Seattle, WA, USA), and incubated with serum free media for 24 hr. Then the cells were treated with FFA and AM as described above, and, another 24 hr later, the … how do we pursue happiness

Has anyone used FL83B cells? ResearchGate

WebJan 1, 2024 · FL83B cells were grown in F12K medium supplemented with 10% foetal bovine serum (FBS)(Biological Industries, Haemek, Israel) and 100 mg/L penicillin and streptomycin at 37 °C in a humidified atmosphere of 5% CO2. Cells induced lipid accumulation in hepatocytes using 0.5 mM oleic acid (OA) for 48 h, then 3–100 μM … WebJul 13, 2024 · Characteristics. tissue/cell type: Liver. condition: Healthy. treatment: none. Treatment protocol. FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non … ph of bone brothWebDec 9, 2024 · FL83B cells were harvested at the exponential growth phase, prepared into a suspension of approximately 1 × 10 6 cells/mL, and 600 μL of this suspension was added to the lower chamber. FL83B cells were stimulated with 40 ng/mL TNF-α for 2 h in model group (+ TNF-α group). FL83B cells were stimulated with an equal volume of PBS for 2 … ph of boiled water

"WebA mouse HCC cell line (Hepa1-6) and a mouse hepatocyte line (FL83B) were used to elucidate the mechanism Cite 6th Dec, 2024 Mohamed Elkablawy Taibah University and … " - Fl83b cells

Fl83b cells

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WebDec 1, 2024 · Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF- α -treated FL83B cells. The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, p -coumaric acid, and cirsimaritin. The bioactive compound with the best effect of increasing glucose uptake was p -coumaric … WebApr 25, 2024 · Briefly, FL83B cells (~ 18 × 10 6) were collected by centrifugation and sonicated in cold diluted assay buffer. The supernatant was removed, and cells were re-suspended in cold diluted assay buffer. BHB standard wells and sample wells were prepared as per the protocol. The reaction was initiated by adding developer solution.

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WebJul 13, 2007 · FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of GRK2 was determined using immunofluoresecence and … WebMay 21, 2024 · Abstract. FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its …

Web(F) Lipid content analysis of FL83B cells treated with 400 mM OA and different CLH concentrations by using Nile red stain. The data are given as mean ± SEM (n ¼ 6). WebDec 1, 2024 · Using the Sephadex LH20 gel packing chromatography column, the EAF5 fraction was isolated from RTL and significantly increased glucose uptake in TNF-α-treated FL83B cells. Moreover, the MCI gel packing chromatography column separated EAF5 into five subfractions and had no significant cytotoxic effect in FL83B cells when treated at …

WebFL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal … WebJul 1, 2014 · FL83B cell morphology before and after DEHP exposure at different concentrations are shown in Fig. 1. With the increase of DEHP concentration, cells tend to shrink. Afterward, the cell lost its ability to adhere on the plate of the dish; thus, it was found floating in the media. Beside shrinkage, cells also showed decrease in cell density.

WebJun 15, 2015 · Abstract We studied the effect of silymarin and dimercaptosuccinic acid (DMSA), a chelating agent that was administered individually or in combination against lead (Pb) toxicity in rats. Wistar rats (200 ± 20) were randomly divided into five groups. Group A served as a control. Groups B–E were exposed to 2000 ppm of lead acetate in drinking …

WebJan 11, 2024 · d FL83B cells were cultured with different concentrations (as indicated) of PC (34:2) for 16 h, and the resulting effects on AhR expression were determined by western blots. Ratio to β-actin ... how do we put ser in the yo formWebApr 1, 1973 · The FL83B cells are morphologically similar to rat liver parenchymal cells [29], to the granular epithelial cells seen on primary explantation of rat liver [30], and to the secondary liver parenchymal cell line obtained by cloning a suspension of rat liver cells [2]. The question of transformation must be addressed in trying to determine the ... ph of borate bufferWebMay 25, 2011 · Finally, the possible mechanism of DPS promoting insulin sensitivity in TNF-α-induced FL83B cells was investigated in this study. DPS may attenuate c-Jun N … ph of bok choyWebAug 1, 2007 · The present study was designed to test the hypothesis that GRK2 negatively regulates IR signaling. FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of ... ph of bore waterWebS-Allyl cysteine attenuates free fatty acid-induced lipogenesis in human HepG2 cells through activation of the AMP-activated protein kinase-dependent pathway ☆ Author links open overlay panel Yong Pil Hwang a b 1 , Hyung Gyun Kim a 1 , Jae Ho Choi a , Minh Truong Do a , Young Chul Chung c , Tae Cheon Jeong d , Hye Gwang Jeong a how do we randomize in a matched pairs designWeb2.3. FL83B cell culture First, the culture method of FL83B cells was slightly modiﬁed from the American Type Culture Collection (ATCC) [18]. The short- and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture sys-tem were determined (Fig. 1A), which could monitor ph of bonesWebCells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and … how do we put on love